|  | | Diatomaceous-based plasmid isolation
- 1ml of
overnight culture
- Add 250ul
of Alkaline Lysis Solution I
- Add 300ul
of 0.2N NaOH/1%SDS, gently mix
- Add 350ul
of 3M K-acetate, gently mix
- Centrifuge
10 min
- Transfer
700ul of supernatant to new tube
- Add 700ul
of 6M Gaumidine HCl
- Add
100ul of 1g/10ml Diatomaceous earth
- Stand
for 5min
- Centrifuge
1 min
- Wash
with 750ul of 50% EtOH in 1XTE
- Centrifuge
- Wash
with 250ul of 50% EtOH in 1XTE
- Centrifuge
- Dry
- Elute
twice with 50ul of 1XTE or ddwater
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